A RE-PCR method to distinguishListeria monocytogenesserovars

PCR optimization: Improving of Human Cytomegalovirus (HCMV) PCR to Achieve a Highly Sensitive Detection Method

Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...

a paradigm shift away from method-wise teaching to strategy-wise teaching: an investigation of reconstructive strategy versus communicative strategy

چکیده: هدف اصلی این مطالعه ی توصیفی تحقیقی در حقیقت تلاشی پساروش-گرا به منظور رسیدن به نتیجه ای منطقی در انتخاب مناسبترین راهکار آموزشی بر گرفته از چارچوب راهبردی مطرح شده توسط والدمر مارتن بوده که به بهترین شکل سازگار و مناسب با سامانه ی آموزشی ایران باشد. از این رو، دو راهکار آموزشی، راهکار ارتباطی و راهکار بازساختی، برای تحقیق و بررسی انتخاب شدند. صریحاً اینکه، در راستای هدف اصلی این پژوهش، ر...

Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes.

Am. J. Pathol. 143:1527-1534. 4.Haase, A.T., E.F. Retael and K.A. Staskus. 1990. Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. USA 87:4971-4975. 5.Janssen, K. (Ed). 1995. In situ polymerase chain reaction and hybridization to detect lowabundance nucleic acid targets, p. 14.8.114.8.24. Current Protocols in Molecular Biology, Vol. 2, Supplement 31. John Wiley ...

TaqMan real-time PCR: a reliable method to detect meat species

The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-t...

Failure of a PCR screening method to detect MRSA.